The role of miR-29 as a mediator of NFAT activation became further evident by decreased NFAT-dependent transcriptional activation of Regulator of calcineurin 1 (Rcan1) in mice injected with antimiR-29 or miR-29-deficient mice after TAC (Supplementary Fig. 5a), and by the finding that addition of the NFAT inhibitor VIVIT to cultured CM prevents miR-29-induced cardiac myocyte hypertrophy (Supplementary Fig. 5b). This evidence concerns the gene RCAN1 and cardiac hypertrophy.