In contrast, transient stroke as short as 0.5 h followed by intravenous treatment at reperfusion disrupted the BBB and increased albumin content in the ipsilateral hemisphere by 24 h post MCAo (observed as positive values after subtraction of the contralateral hemisphere) in both vehicle and rt-PA-treated mice, an effect which was dramatically amplified by 29- and 33.1-fold, respectively, when stroke duration was extended to 4 h (p < 0.0001, Fig. 1c). Here, ALB is linked to stroke disorder.