In addition, using immunoblotting, immunofluorescence staining, and RT-PCR analysis, a study by Cocchiola et al. demonstrated that compared with the matched normal sections, prostate tumors with low Gleason scores (i.e. 6-7) exhibited a nuclear expression of pY705-STAT3 protein, whereas prostate tumors with high Gleason cases (i.e. 8–9) showed a cytoplasmic presence of pY705-STAT3 [30]. The gene discussed is STAT3; the disease is prostate neoplasm.