Here, we further identified and characterized a new PTEN-COL17A1 fusion gene in human gliomas, our results demonstrated that Collagen XVII promoted cell invasion through MMP9-mediated hydrolysis of the cell matrix, and revealed a novel regulatory role of PTEN-COL17A1 fusion in Collagen XVII expression and GBM malignance. The gene discussed is PTEN; the disease is glioma.