To explore the role of PVT1 in renal cancer cells, we stably regulated the expression of PVT1 in two RCC cell lines 786-O and ACHN with lenti-viral system, using short hairpin RNA (shRNA) or full-length PVT1 plasmid to silence or overexpress the PVT1 respectively. The gene discussed is PVT1; the disease is renal carcinoma.