MYCN and colon adenocarcinoma: We included 1 medulloblastoma and 1 colon adenocarcinoma cell line, each reported to harbor a MYC amplification [36, 37], to test the specificity with which our ddPCR protocol detects MYCN. We extracted gDNA from this cell line panel, then sheared gDNA samples by sonication prior to ddPCR copy number detection.