We included 1 medulloblastoma and 1 colon adenocarcinoma cell line, each reported to harbor a MYC amplification [36, 37], to test the specificity with which our ddPCR protocol detects MYCN. We extracted gDNA from this cell line panel, then sheared gDNA samples by sonication prior to ddPCR copy number detection. Here, MYCN is linked to medulloblastoma.