MYCN and colon adenocarcinoma: We measured 1.85 ± 0.11 MYCN copies in gDNA from the HD-MB03 medulloblastoma cell line and 1.67 ± 0.17 MYCN copies in gDNA from the COLO-320 colon adenocarcinoma cell line (Figure 4, Table 1), confirming the specificity of our ddPCR assay for MYCN. In conclusion, we confirmed the MYCN copy number status reported for most neuroblastoma cell lines examined in our panel, and provide evidence for a MYCN gain in the SH-SY5Y cell line and a strong MYCN amplification in the NB-1 cell line.