In addition, in both PANC-1 and Capan-2 orthotopic xenograft tumor tissues, brusatol combined with GEM or 5-FU group showed stronger staining for E-cadherin, and weaker Twist staining when compared with the chemotherapeutic agent alone and the control group, and the combination treatment was observed to produce the most obvious effect (P < 0.01). The gene discussed is TWIST1; the disease is neoplasm.