To precisely identify a specific site on Smad3, that is targeted in HCC by microRNA-145, a thorough search on MicroRNA database coupled with use of computational methods (MiRanda, TargetScan, PicTar) pointed to a locus on Smad3 gene which codes for proteins involved in immunosuppression and anti-inflammatory responses mediated by TGF-β [37]. The gene discussed is TGFB1; the disease is hepatocellular carcinoma.