Everolimus is relatively well tolerated with long-term treatment as has been shown in the EXIST-1 study (NCT00789828), where everolimus was used to treat tuberous sclerosis patients (germ-line mutations in TSC1/2) with subependymal giant cell astrocytomas (SEGAs) [58]. This evidence concerns the gene TSC1 and astrocytoma (excluding glioblastoma).