We exposed these patient-derived GBM lines to our MTH1 inhibitors TH588 and TH1579 and observed that the inhibition of MTH1 by either compound significantly decreased viability of all GBM cultures, the improved MTH1 inhibitor TH1579 being more potent compared to the parental compound TH588 (Figure 2A, 2B, Supplementary Figure S3). Here, NUDT1 is linked to glioblastoma.