Ancestor clones evolved along three different trajectories: clade 1 fostered metastatic clones that displayed some metastatic-related genomic alterations, such as PIK3CA and ARHGAP26 mutations, as well as chromosome 13 arm-level deletion; clade 2 fostered branch-specific subclones, characterized by branch-specific alterations such as JAK1 mutations and PTEN deletion; clade 3 represented a vertical region of tumor cell transmission from the primary tumor [185]. Here, PIK3CA is linked to neoplasm.