As shown in our data, patients with theFVIIImutations exhibited significantly lower expression of theFVIIImRNA, which may be translated with the reduced levels of FVIII procoagulant activity in situ around the tumors (Fig. 5), although immunohistochemical studies did not detect the FVIII protein in all seven cases of melanoma (Fig. 5andTable 2). This evidence concerns the gene F8 and melanoma.