The most upregulated gene was PMEPA1 (prostate transmembrane protein androgen induced-1), which was part of a negative feedback loop and served as a TGF-β signaling regulator to suppress PCa metastases to bone, via interacting with SMAD2/3 and HECT E3 ubiquitin ligases [103]. This evidence concerns the gene TGFB1 and posterior cortical atrophy.