To determine the role of Necdin in AML-ETO9a -induced leukemia, we transplanted 100,000 wild type or Necdin null HSPCs expressing AML-ETO9a (CD45.2+GFP+) into lethally irradiated recipient mice (B6.SJL mice, CD45.1+) together with 100,000 normal competitor cells (CD45.1+). Here, NDN is linked to leukemia.