Our current study further investigated 13 commonly used colon cancer cell lines and two isogenic cell lines with heterozygous knock-in of either of PIK3CA mutations c.3140A>G (p.H1047R) and c.1633G>A (p.E545K), both of which are commonly observed in colorectal carcinoma. The gene discussed is PIK3CA; the disease is malignant colon neoplasm.