We evaluated the effects of aspirin on induction of apoptosis and cell cycle distribution in three PIK3CA-mutant cell lines (HCT15, HCT116, and SW948) that were particularly sensitive to aspirin exposure in the MTS assay and three PIK3CA-wild-type colon cancer cell lines (COLO205, SW480, and SW620) that were resistant to aspirin exposure. Here, PIK3CA is linked to malignant colon neoplasm.