Based on these assumptions, we analyzed RhoC protein expression as well as RhoA protein expression in human tissue specimens of matched normal epithelium, ductal carcinoma in situ (DCIS) and ductal breast carcinomas of 9 cases (with 1/9 cases pTis, pNx; 8/9 cases with pT1b to pT2 and pN0 to pN2a) by immunohistochemistry. The gene discussed is RHOA; the disease is breast ductal adenocarcinoma.