After co‐confecting Ad‐PGRN‐shRNA and the miR‐34b‐5p antagomir in vivo, the miR‐34b‐5p antagomir displayed similarly protective effects in the groups without PGRN inhibition, further confirming that miR‐34b‐5p inhibition was useful for ALI. Here, GRN is linked to acute respiratory distress syndrome.