Lentiviral transduction of GFI1-eGFP containing vectors into KG-1, THP-1, K-562 and Kasumi-1 human AML cell lines inhibited their clonogenic capacity in semi-solid medium (Fig. 3a) as indicated by smaller colonies and cell numbers compared to control eGFP-transduced cells (Fig. 3b,d–k). Here, GFI1 is linked to acute myeloid leukemia.