Phosphoproteomic approaches have demonstrated effective identification of the abnormal protein kinase activity involved in cancer.21,22 Using NSCLC as a proof-of-concept study, label-free quantitative phosphoproteomic analysis of a pair of cancerous and its adjacent normal tissues revealed site-specific elevated phosphorylated proteins/peptides in NSCLC. The gene discussed is WEE1; the disease is non-small cell lung carcinoma.