To compare and potentially validate distinct protein expression patterns across GBM TMEs based on 5-ALA guided sampling, we performed a similar analysis of 76 IDH wild type (wt) WHO grade II/III gliomas and 136 IDH-wt primary GBMs (WHO grade IV) using 176 proteins from the TCGA RPPA database. The gene discussed is IDH1; the disease is central nervous system cancer.