TLR4 and liver disorder: Viewed from the perspective of the circulating concentrations of all sizes of hyaluronan both in health and liver disease, it is unlikely that concentrations of endogenous specific-sized HA35 would likely have an impact on TLR4 signaling, as purified HA35 was used at a pharmacological concentration of 100 grams/L (100 micrograms/ml) in our cell based studies.