We performed a preliminary screen for expression levels of FSCN1 in four mammary carcinoma cell lines (contains one of the non-TNBC cell line MCF-7, and three TNBC cell lines MDA-MB-468, MDA-MB-231 and MDA-MB-453) by Real-Time Polymerase Chain Reaction (PCR) (Fig. 3A). This evidence concerns the gene FSCN1 and breast carcinoma.