BLI generated by three breast cancer cell lines (ER+ MCF-7, ER-/Her2+ SK-BR-3, and ER- MDA-MB-231) at 24, 48, 72, and 96 h of culture in the presence of control medium (DMEM-10%FBS) vs. bone tissue-conditioned media from THRs 133, 129, 147, 122, and 116. The gene discussed is ESR1; the disease is breast carcinoma.