A BLI signal generated by six breast cancer cell lines (ER+ MCF-7, ZR-75 and T-47D, ER-/Her2+ SK-BR-3, and ER- MDA-MB-231 and MCF-10A) at 24, 48, 72, and 96 h of culture in plastic wells vs. bone tissue fragments isolated from THR 151. The gene discussed is ESR1; the disease is breast cancer.