To compare intracellular Ca2+ influx after TRPV3 activation between keratinocytes cultured from normal tissue and burn-scarred tissue with or without pruritus, TRPV3 agonist (500 μM Carvacrol and 200 μM 2-APB mixture) was used to treat each group. The gene discussed is TRPV3; the disease is Pruritus.