F2RL1 and Pruritus: To examine the mechanism of amplified TRPV3 activation in PAR2 agonist pretreated keratinocytes of burn-scar tissue, we evaluated the level of intracellular Ca2+ of cultured keratinocytes from normal tissue or burn-scarred tissue (with or without pruritus) after inhibiting PLC-β, protein kinase A (PKA) and protein kinase C (PKC), which are essential components of intracellular Ca2+ influx signalling by PAR2.