G6PD and neoplasm: However, supplementation of nucleosides mix in medium raised the cellular EdU incorporation in cells expressing G6PD 406A and 466A mutants to that of the WT G6PD-expressing cells (Supplementary Fig. 6d), further underlining the importance of macromolecular synthesis via G6PD phosphorylation and activation for Plk1-mediated cell cycle progression in tumor cells.