G6PD and neoplasm: Intriguingly, supplementation of NAC and Nuc mixtures in medium reduced the duration time of mitotic cells expressing T406A, T406D, and T466A to that of the G6PD WT or T466D mutant, underlining the importance of macromolecular synthesis via Plk1-mediated G6PD phosphorylation and activation during the cell cycle progression of tumor cells (Fig. 6b).