TF and neoplasm: Forty-six somatic substitutions, including 15 novel variants, were identified in 35 out of 86 tumor tissues and were absent in the matched non-tumor samples; of those, 21 substitutions were distributed in the D-loop (14), 12S rRNA (2), 16S rRNA (1), and tRNAs (4, in the MT-TF, MT-TN, MT-TS2, and MT-TP genes) (Table 2).