TNS4 and metastatic malignant neoplasm in the lymph nodes: As a result, we demonstrated that CTEN was overexpressed in 48% (29/60) of primary AEG tissues and 57% (4/7) of cell lines, and CTEN overexpression was significantly correlated with more aggressive venous and lymphatic invasion, deeper tumor depth, and higher rates of lymph node metastasis and recurrence.