TNS4 and gastric cancer: A formalin-fixed gastric cancer MKN45 cell line presenting the overexpression of CTEN, in which > 50% of cells showed staining, was used as a positive control, whereas a formalin-fixed gastric cancer HGC27 cell line (data not shown) and MKN45 staining without the CTEN antibody presenting low expression of CTEN was included as a negative control (Figure 1C).