MAP2K1 and non-small cell lung carcinoma: To further investigate whether SNHG12-miR-181a-MAPK/Slug regulatory axis existed in NSCLC cells, the levels of MAPK1, p-MAPK1, MAP2K1, p-MAP2K1 and Slug were determined by western blot analysis in A549 and A549/DDP cells that were transfected with (pcDNA-SNHG12 or pcDNA-SNHG12-Mut) or anti-miR-181a or si-SNHG12-1 or co-transfected with si-SNHG12-1 and miR-181a mimics.