In particular, when 70 CRC samples were assessed using ISAD-KRAS, 5 cases (no. 13, 15, 18, and 68 for G12D and no. 5 for G13D) were found to have a mutation, which could not be detected by PCR and direct sequencing (Supplementary Table 3, Figure 4B). The gene discussed is KRAS; the disease is colorectal carcinoma.