As an example, it has been reported that maximal DNA de-methylation was achievable after 48 hr treatment with DNMT inhibitors, 5-azacytidine or 2’-deoxy-5-azacytidine (decitabine) [50, 51] and the mRNA expression of p16 TS gene was detected beginning 36 hr after decitabine-treatment in T24 bladder carcinoma cells [51]. This evidence concerns the gene CDKN2A and urinary bladder carcinoma.