Whether Wnt/β-catenin signaling pathway is involved in the mechanism of miR-27a-3p targeting RXRα in CRC progression, our data showed that miR-27a-3p inhibitor dramatically increased RXRα and suppressed β-catenin, Frizzled-7, Dvl2, Dvl3, p-LRP6, Axin1, and GSK3β expression in HCT116 cells compared with the control group. The gene discussed is AXIN1; the disease is colorectal carcinoma.