Western blot analysis indicated that the nuclear PATZ1 expression in Nthy-ori 3-1 cells was higher than that in thyroid cancer cell lines, and the nuclear PATZ1 expression in TPC-1 that originated from DTC was higher than that in the ATC cell lines (ACT-1 and FRO) (Figure 2B). This evidence concerns the gene PATZ1 and thyroid gland carcinoma.