PATZ1 and thyroid gland disorder: First, to confirm that the cell lines used in the study maintained the same characteristics regarding PATZ1 as clinical thyroid specimens, we examined PATZ1 cellular localization in an immortalized normal thyroid epithelial cell line, Nthy-ori 3-1, and four thyroid cancer cell lines (TPC-1, FTC-133, FRO, and ACT-1) by immunofluorescence (Figure 2A).