As shown in Figure 2, most of the differentially expressed genes in NAFLD vs normal group had the opposite expression patterns in LGZG vs NAFLD group, e.g. Pik3r1, Forkhead O transcription factors 1 (Foxo1), stearoyl CoA desaturase 1 (Scd1) were up-regulated in NAFLD rats, whereas down-regulated in LGZG group. This evidence concerns the gene PIK3R1 and metabolic dysfunction-associated steatotic liver disease.