In conclusion, our study demonstrates that, (1) miR-493-5p is downregulated in prostate cancer possibly due to DNA methylation; (2) miR-493-5p acts as a tumor suppressor in prostate cancer; (3) c-Met, CREB1 and EGFR are miR-493-5p target genes; (4) miR-493-5p suppresses EMT by inhibiting AKT/GSK-3β/Snail signaling pathway in prostate cancer; and (5) CREB1 regulates c-Met/Akt/GSK-3β/Snail signaling pathway indirectly. The gene discussed is CREB1; the disease is neoplasm.