TP53 and carcinoma: To further investigate the binding affinity of TAp63 and TAp73 towards 11 different mutant forms of p53, we transfected p53-null human carcinoma H1299 cell line [92] with wild type (WT) or mut p53 and performed co-immunoprecipitation of p53 with ectopically expressed TAp63α or TAp73α (Figure 2A, 2B).