According to Western blot, the expression of E-cadherin, p-NF-кB, Akt, p-Akt, p53, Cdk4, Cdc2, AIF, ADFP and MRP1 was up-regulated, but the expression of N-cadherin, Twist, snail, Zeb1, Slug, VEGF, Claudin-1, Cyclin B1, c-myc and FBXW7 was down-regulated in ING5 transfectants of both breast cancer cells (Figure 2B). This evidence concerns the gene TWIST1 and breast cancer.