The ectopic expression of TLX1, however, does not induce any significant increase in the already high levels of ICN1 present in VAV1−/− Jurkat cells (Figures S8B and S8C), further indicating that the effect of TLX1 on ICN1 abundance in WT cells is directly linked to the repression of VAV1. Conversely, we found that knockdown of endogenous TLX1 in TLX1+ ALL-SIL cells promotes expression of endogenous Vav1 and reduces ICN1 (Figures 8B and S8B). Here, VAV1 is linked to acute lymphoblastic leukemia.