The increase in Notch1 mRNA abundance is seen using primers for both the 5′ and 3′ end of its cDNA (Figure 2F), indicating enhanced transcription from the WT locus rather than spurious expression of an ICN1-encoding mRNA found in some T-ALL (Jeannet et al., 2010). This evidence concerns the gene NOTCH1 and acute lymphoblastic leukemia.