The nuclear speckle protein SRSF2/SC-35 displayed a nuclear punctate staining pattern in all subjects, with some ALS cases showing strong and larger speckles, while one SALS case also exhibited cytoplasmic SC-35 inclusions that did not co-localize with TDP-43 (Figs. 1b, 4b, white arrowheads). The gene discussed is TARDBP; the disease is amyotrophic lateral sclerosis.