Given that AML-like tumours derived from our engineered cells comprise multiple different types of cells, as well as the previous proposal that AMLs arise from a neural crest-derived cell type5, we cultured shRNA-Tsc2/Cdkn2a∆/∆ sphere cells in specific adipocyte-, osteoblast-, neural crest- and epithelial- differentiation media to assess their capacity to form different cellular lineages in culture. The gene discussed is TSC2; the disease is neoplasm.