CD4 and amyotrophic lateral sclerosis: Also, flow cytometry analysis and subsequent proliferative response assay of peripheral blood monocytes obtained from ALS patients 4 h and 24 h after infusion of MSCs, showed a dramatic increase in CD4+ CD25+ regulatory T cells, coupled with a reduction in activated dendritic cells and lymphocyte proliferation, demonstrating the immediate immunomodulatory properties of MSCs following transplantation [80].