RNA-Seq results for EGFRvIII-upregulated and TRIM24 shRNA-downregulated genes were validated by measuring mRNA levels on a subset of six selected genes involved in tumor growth by quantitative RT-PCR (qRT-PCR) analyses, including Inhibitor of DNA binding 1 (ID1), Inhibitor of DNA binding 3 (ID3), Fibroblast growth factor receptor 3 (FGFR3), Transforming growth factor alpha (TGFA), Aurora kinase A (AURKA) and DexD-box helicase 39 A (DDX39A) (Supplementary Fig. 4). The gene discussed is ID3; the disease is neoplasm.