Here, we show that although TRIM24 does not directly bind to the NF-κB p65 subunit, TRIM24 significantly altered the expression of NF-κB target genes in EGFRvIII-driven GBM cells, suggesting that TRIM24 regulates NF-κB in GBM cells in a manner distinct from STAT3. This evidence concerns the gene STAT3 and glioblastoma.