Importantly, shRNA knockdown of endogenous TRIM24 in LN229 and U87 GBM cells had no effect on the expression of EGFR, EGFRvIII, or β-actin, thus excluding off-target effects of the shRNA constructs and confirming our hypothesis that TRIM24 is a downstream target of EGFR signaling (Fig. 3a). This evidence concerns the gene TRIM24 and glioblastoma.