PKM and hilar cholangiocarcinoma: In a study based on 16 months median follow-up of 88 hilar cholangiocarcinoma patients, HK1 and PKM2 levels were found to be higher in human hilar cholangiocarcinoma tumours than in normal tissue samples, and this expression was correlated with lymph node metastasis, tumour differentiation, TNM stage and poor prognosis32.