Intrahepatic metastasis (HR = 10.985, P = 0.033), TNM stage (HR = 1.295, P = 0.039), vascular invasion (HR = 1.116, P = 0.036) and PKM2 expression (HR = 4.100, P = 0.027) were adverse prognostic factors affecting RFS in non-cirrhotic HCC after resection (Table 3). This evidence concerns the gene PKM and hepatocellular carcinoma.