Finally, 18 SMGs were identified (Fig. 2, Supplementary Table 6), 15 of which had been previously reported in ESCC (TP53, AJUBA, CDKN2A, KMT2D(MLL2), ZNF750, FAT1, NOTCH1, NOTCH3, PIK3CA, NFE2L2, RB1, KDM6A, FBXW7, CREBBP, and TGFBR2). This evidence concerns the gene TP53 and esophageal squamous cell carcinoma.