To verify this possibility and exam the biological impact of HDAC7 depletion on human lung cancer cells, we silenced HDAC7 expression by lentivirus-mediated shRNA in two human lung cancer cell lines (H1299 and H522) with wt K-RAS and two human lung cancer cell lines (A549 and H2009) with a mutant K-RAS to mimic the conditions of our mouse study (Fig. 3a and Additional file 1: Figure S1B). Here, KRAS is linked to lung carcinoma.