The expression levels of several hepatic fibrosis markers such as alpha smooth muscle actin (αSMA), collagen type 1 alpha 1 chain (Col1A1), fibronectin 1 (FN1), PCNA and tissue inhibitor of metalloprotease 1 (TIMP1) were tested by RT-qPCR (Figure 9). This evidence concerns the gene TIMP1 and Hepatic fibrosis.