This yielded 88 genes associated with tumor grade, in which seven DEGs (C8orf33, TSNAXIP1, TM4SF1, CTH, ANXA2, KIAA1522 and LRRC1) can distinguish HCC of G3 from G1, two DEGs (CYB5A and RRAGD) can distinguish HCC of G3 from G2, and two DEGs (CFHR4 and F13B) can distinguish HCC of G3 from G4 (Fig. 4). The gene discussed is NHSL3; the disease is hepatocellular carcinoma.