Hippocampal neurons transfected with VGLUT2-pH at the time of plating were infected at DIV 7 with lentivirus containing an shRNA oligonucleotide targeted to AP-1γ or AP-3δ1, along with blue fluorescent protein as a reporter to measure infection efficiency, as described (Foss et al., 2013; Santos et al., 2013). The gene discussed is AP3D1; the disease is infection.