Additionally, Nec-1, which inhibits necroptosis, reduced increased necrotic cells and LDH leakage rate after OGD/R injury from 80.84 ± 2.00 and 51.69 ± 1.62% to 39.24 ± 0.96 and 22.76 ± 2.75%, indicating that necroptotic neuronal death occurs in primary neurons following ischemia in vitro. Here, PCSK1 is linked to ischemia.