NFKB1 and vitamin D deficiency: We aimed to address these limitations by examining the effects of vitamin D supplementation on inflammatory markers and NFκB activity in overweight or obese but otherwise healthy individuals, with vitamin D-deficiency (25(OH)D ≤ 50 nmol/L), who receive a sufficient dose (≥4000 IU daily) of supplementation, in a clinical trial without co-interventions and with rigorous methods to minimize confounding by lifestyle factors.