In the vaccinated mice challenged with Mab, the two groups with Mpg in the vaccine module, groups III [BCG + Mpg] and IV [Mpg + Mpg], showed a significantly more robust IFN-γ secretion and yielded significantly higher numbers of IFN-γ spot forming cells (SFCs) compared to groups I [PBS + PBS] or II [BCG + BCG] at 3 or 14 days after infection. The gene discussed is IFNG; the disease is infection.